rat anti mouse cd4 fitc  (SouthernBiotech)

Journal: Dose-Response

Article Title: Comparative Analysis of Concurrent vs Sequential Administration of anti-PD-1 Following Thoracic Radiotherapy: Impact on Lung Tissue Damage

doi: 10.1177/15593258251322324

Figure Lengend Snippet: Recruitment of inflammatory infiltration cells in the lung tissue. (A) T lymphocytes infiltrated lung tissue in different groups. Green fluorescence: CD4 + T cells, red fluorescence: CD8 + T cells, and DAPI: blue fluorescence. (B) Infiltrating macrophages in lung tissue in different groups. (C) Infiltrating neutrophils in lung tissue in different group. * P < .05, ** P < .01.

Article Snippet: The collected BALF cells were stained 30 min at 4°C in 0.2% bovine serum albumin in PBS containing 1:100 dilution of the following antibodies: APC-Cy7 anti-mouse CD45 (BD Biosciences, cat. no. 557659), APC anti-mouse CD3 (CST, cat. no. 24265), FITC anti-mouse CD4 (CST, cat. no. 96127), BV605 anti-mouse CD8 (BD Biosciences, cat. no. 563152), PE anti-mouse CD11b (CST, cat. no. 24965), BV421 anti-mouse F4/80 (BD Biosciences, cat. no. 565411), PerCP-cy5.5 anti-mouse ly6G (CST, cat. no. 63460).

Techniques: Fluorescence